基因沉默
ADAM10型
癌症研究
生物
癌基因
基质凝胶
转移
胰腺癌
癌症
癌细胞
活力测定
细胞周期
分子医学
去整合素
细胞
金属蛋白酶
血管生成
基质金属蛋白酶
基因
生物化学
遗传学
作者
Matthias M. Gaida,Natascha Haag,Frank Günther,Darjus F. Tschaharganeh,Peter Schirmacher,Helmut Friess,Nathalia A. Giese,Jan Schmidt,Moritz N. Wente
标识
DOI:10.3892/ijmm_00000463
摘要
The protease ADAM10 influences progression and metastasis of cancer cells and is overexpressed in various malignancies. Therefore, the aim of our study was to evaluate the expression and potential function of ADAM10 in the pathophysiology of pancreatic cancer (PDAC). ADAM10 expression in normal pancreatic (NP), chronic pancreatitis (CP), PDAC tissues, as well as PDAC cell lines was determined. To evaluate whether rhADAM10 or ADAM10 silencing influences cancer cell viability, MTT assay was used. Matrigel invasion and wound healing assays were performed to observe influence on invasion and migration. ADAM10 mRNA was expressed in all samples of NP, CP and PDAC tissue and cell lines. Western blotting and immunohistochemistry revealed stronger ADAM10 expression in PDAC than in NP. ADAM10 silencing or rhADAM10 had no effect on cell viability. ADAM10 silencing markedly reduced invasiveness and migration of cancer cells. These findings establish ADAM10 as a contributing factor in PDAC invasion and metastasis.
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