反平行(数学)
螺旋束
螺旋线圈
捆绑
螺旋(腹足类)
蛋白质设计
结晶学
单体
α螺旋
化学
蛋白质结构
物理
材料科学
圆二色性
生物
生物化学
聚合物
蜗牛
有机化学
复合材料
磁场
量子力学
生态学
作者
Po-Ssu Huang,Gustav Oberdorfer,Chunfu Xu,X.Y. Pei,Brent L. Nannenga,Joseph M. Rogers,Frank DiMaio,Tamir Gonen,Ben F. Luisi,David Baker
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2014-10-24
卷期号:346 (6208): 481-485
被引量:260
标识
DOI:10.1126/science.1257481
摘要
We describe a procedure for designing proteins with backbones produced by varying the parameters in the Crick coiled coil-generating equations. Combinatorial design calculations identify low-energy sequences for alternative helix supercoil arrangements, and the helices in the lowest-energy arrangements are connected by loop building. We design an antiparallel monomeric untwisted three-helix bundle with 80-residue helices, an antiparallel monomeric right-handed four-helix bundle, and a pentameric parallel left-handed five-helix bundle. The designed proteins are extremely stable (extrapolated ΔGfold > 60 kilocalories per mole), and their crystal structures are close to those of the design models with nearly identical core packing between the helices. The approach enables the custom design of hyperstable proteins with fine-tuned geometries for a wide range of applications.
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