下调和上调
Wnt信号通路
生物
诱导多能干细胞
血管紧张素转化酶2
细胞生物学
心肌细胞
胚胎干细胞
心脏发育
扩张型心肌病
心肌病
肾素-血管紧张素系统
细胞
细胞培养
心力衰竭
信号转导
基因
内科学
内分泌学
医学
遗传学
2019年冠状病毒病(COVID-19)
传染病(医学专业)
血压
疾病
作者
Xiuli Shao,Xiaolin Zhang,Ruijia Zhang,Rongli Zhu,Xiuyang Hou,Weijue Yi,Fang Wu,Hao Liang,Rui Feng
出处
期刊:American Journal of Physiology-cell Physiology
[American Physiological Society]
日期:2022-04-01
卷期号:322 (4): C723-C738
被引量:4
标识
DOI:10.1152/ajpcell.00169.2021
摘要
Numerous studies have shown that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect host cells through binding to angiotensin I converting enzyme 2 (ACE2) expressing in various tissues and organs. In this study, we deeply analyzed the single-cell expression profiles of ACE2 in fetal and adult human hearts to explore the potential mechanism of SARS-CoV-2 harming the heart. The molecular docking software was used to simulate the binding of SARS-CoV-2 and its variant spike protein with ACE2. The genes closely related to ACE2 in renin-angiotensin system (RAS) were identified by constructing a protein-protein interaction network. Through the analysis of single-cell transcription profiles at different stages of human embryos, we found that the expression level of ACE2 in ventricular myocytes was increased with embryonic development. The results of single-cell sequencing analysis showed that the expression of ACE2 in ventricular myocytes was upregulated in heart failure induced by dilated cardiomyopathy compared with normal hearts. The upregulation of ACE2 increases the risk of infection with SARS-CoV-2 in fetal and adult human hearts. We also further confirmed the expression of ACE2 and ACE2-related genes in normal and SARS-CoV-2-infected human pluripotent stem cell-derived cardiomyocytes. In addition, the pathway analysis revealed that ACE2 may regulate the differently expressed genes in heart failure through calcium signaling pathway and Wnt signaling pathway.
科研通智能强力驱动
Strongly Powered by AbleSci AI