肠道固有淋巴样细胞亚群分类及表型检测

40% percoll 80% percoll 4 ml 40% percoll 15 ml 3 ml 2.5 ml 80% percoll 15 ml percoll800 g 20 min (ACC) 1 (DEC) 0 16. 10 ml PBS 800 x g