The relationships between the diffuse reflectance of tissue and its optical parameters (especially the refractive index and the ratio N' of effective scattering coefficient to absorption coefficient) are studied by Monte Carlo method. The limitation in the diffuse reflectance formula fitted by others is found, and a new diffuse reflectance formula which is different for N' in different region is presented. And the diffuse reflectance formulas are obtained for N' = 2 ~ 20 and N' = 40 ~ 100, respectively.