抗体依赖性细胞介导的细胞毒性
生物
细胞毒性T细胞
免疫学
抗原
免疫系统
CD16
CD8型
淋巴细胞
抗体
过继性细胞移植
Fc受体
单克隆抗体
T细胞
癌症研究
CD3型
体外
生物化学
作者
Béatrice Clémenceau,Nicolas Congy‐Jolivet,Géraldine Gallot,Régine Vivien,Joëlle Gaschet,Gilles Thibault,Henri Vié
出处
期刊:Blood
[American Society of Hematology]
日期:2006-06-15
卷期号:107 (12): 4669-4677
被引量:77
标识
DOI:10.1182/blood-2005-09-3775
摘要
Abstract In the context of transplantation, donor and virus-specific T-lymphocyte infusions have demonstrated the dramatic potential of T cells as immune effectors. Unfortunately, most attempts to exploit the T-cell immune system against nonviral malignancies in the syngeneic setting have been disappointing. In contrast, treatments based on monoclonal antibodies (Abs) have been clinically successful and have demonstrated the clinical relevance of several antigens as therapeutic targets and the importance of the antibody-dependent cellular cytotoxicity (ADCC) pathway. In the present study, we considered the possibility of arming specific T cells with a receptor that would enable them to mediate ADCC. After transduction with a CD16/γ receptor gene, CD4+ and CD8+ cytotoxic T lymphocytes displayed stable expression of the CD16 receptor at their surface. In the absence of Ab, CD16/γ expression did not affect the capacity of specific T lymphocytes to kill their target following “natural” T-cell receptor recognition. When tested against the autologous B-lymphoblastoid cell line (BLCL) coated with anti-CD20 mAb, the newly expressed Fc receptor enabled the T cells to kill the BLCL through ADCC. Adoptive transfer of such newly designed immune effector may be considered to increase antibody efficiency by harnessing the immune potential of T cells.
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