生物
上皮细胞粘附分子
细胞生物学
干细胞
细胞
干细胞标记物
细胞粘附分子
遗传学
作者
Masaru Okabe,Yusuke Tsukahara,Minoru Tanaka,Kaori Suzuki,Shigeru Saito,Yoshiko Kamiya,Tohru Tsujimura,Koji Nakamura,Atsushi Miyajima
出处
期刊:Development
[The Company of Biologists]
日期:2009-06-01
卷期号:136 (11): 1951-1960
被引量:262
摘要
Hepatic oval cells are considered to be facultative hepatic stem cells(HSCs) that differentiate into hepatocytes and cholangiocytes in severely injured liver. Hepatic oval cells have also been implicated in tumorigenesis. However, their nature and origin remain elusive. To isolate and characterize mouse oval cells, we searched for cell surface molecules expressed on oval cells and analyzed their nature at the single-cell level by flow cytometric analysis and in the in vitro colony formation assay. We demonstrate that epithelial cell adhesion molecule (EpCAM) is expressed in both mouse normal cholangiocytes and oval cells, whereas its related protein, TROP2, is expressed exclusively in oval cells, establishing TROP2 as a novel marker to distinguish oval cells from normal cholangiocytes. EpCAM+ cells isolated from injured liver proliferate to form colonies in vitro, and the clonally expanded cells differentiate into hepatocytes and cholangiocytes,suggesting that the oval cell fraction contains potential HSCs. Interestingly,such cells with HSC characteristics exist among EpCAM+ cells of normal liver. Intriguingly, comparison of the colony formation of EpCAM+ cells in normal and injured liver reveals little difference in the number of potential HSCs, strongly suggesting that most proliferating mouse oval cells represent transit-amplifying cells rather than HSCs.
科研通智能强力驱动
Strongly Powered by AbleSci AI