衰老
细胞分裂素
生物
基因
细胞生物学
转基因
限制
基因表达
遗传学
生长素
机械工程
工程类
作者
Susheng Gan,Richard M. Amasino
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1995-12-22
卷期号:270 (5244): 1986-1988
被引量:1023
标识
DOI:10.1126/science.270.5244.1986
摘要
Controlling expression of IPT, a gene encoding isopentenyltransferase (the enzyme that catalyzes the rate-limiting step in cytokinin biosynthesis), with a senescence-specific promoter results in the suppression of leaf senescence. Transgenic tobacco plants expressing this chimeric gene do not exhibit the developmental abnormalities usually associated with IPT expression because the system is autoregulatory. Because sufficient cytokinin is produced to retard senescence, the activity of the senescence-specific promoter is attenulated. Senescence-retarded leaves exhibit a prolonged, photosythetically active life-span. This result demonstrates that endogenously produced cytokinin can regulate senescence and provides a system to specifically manipulate the senescence program.
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