壁酰二肽
脂磷壁酸
促炎细胞因子
CD80
生物
CD86
CD14型
细胞生物学
树突状细胞
MHC II级
T细胞
抗原呈递
免疫系统
免疫学
CD40
生物化学
炎症
体外
金黄色葡萄球菌
细胞毒性T细胞
细菌
遗传学
作者
Hye Jin Kim,Jae Seung Yang,Sang Su Woo,Sun Kyung Kim,Cheol‐Heui Yun,Kack Kyun Kim,Seung Hyun Han
摘要
Abstract Maturation is an important process by which dendritic cells (DC) develop the potent antigen-presentation capacity necessary for efficient activation of adaptive immunity. Here, we have investigated the ability of lipoteichoic acid (LTA) and muramyl dipeptide (MDP; the minimal structural unit of peptidoglycan with immunostimulating activity) to induce maturation of human immature DC (iDC), derived from peripheral blood CD14-positive cells, and the production of proinflammatory cytokines. Exposure of iDC to staphylococcal LTA (StLTA) at 1 or 10 μg/ml or MDP at 0.1 or 1 μg/ml alone had little effect on the expression of CD80 and CD83, with a minor increase in expression of CD86, all of which are indicative of cell surface markers for maturation. However, there was a synergistic expression of these molecules when iDC were stimulated with StLTA and MDP together. It is interesting that selective induction of MHC Class II expression was observed during the DC maturation, only when costimulated with LTA plus MDP, and Escherichia coli LPS induced dramatic expression of MHC Classes I and II. Endocytosis assay using Dextran-FITC showed that costimulation with StLTA and MDP attenuated the endocytic capacity of the DC, which is a typical phenomenon of DC maturation. Concomitantly, increased expression of DEC-205, but decreased expression of CD206, was observed under the same costimulating condition. Furthermore, ELISA showed that secretions of TNF-α and IL-12 p40, but not IL-10, were induced in iDC by the costimulation. These results suggest that StLTA and MDP synergistically induce maturation and activation of human DC.
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