In order to reveal the change rule of vibration acceleration during micro-end-milling straight groove, the single-factor and orthogonal experiments are employed to confirm the influence of milling parameters on vibration acceleration. On this basis, the relation equation between milling parameters and vibration accelerations is built. The result show that, the vibration acceleration along the width of groove is bigger than that of feed direction and it will increase rapidly with the improve of cutting speed; the predicted result of vibration acceleration is in good accordance with test result, therefore the relation equation can be used to predict vibration acceleration.