Simultaneous Determination of Ginsenoside Rg_1 and Ginsenoside Re in Total Saponins of Fibrous Roots of Panax notoginseng by HPLC-MS/MS

OBJECTIVE: To establish a HPLC-MS/MS method for simultaneous determination of ginsenosides Rg1 and ginsenosides Re in total saponins of fibrous roots of Panax notoginseng. METHODS: BDS HYPERSUL C18(150 mm×2.1 mm,5 μm) column was used with mobile phase consisted of acetonitrile-water (gradient elution). The column temperature was set at 30 ℃. The ginsenosides Rg1 and ginsenosides Re were detected by the negative electrospray ionization mode. Ion pair of reference substance in quantitative determination were as follows: Rg1: m/z 799.6→475.5; Re:m/z 945.8→783.7; paclitaxel: m/z 852.5→525.3. RESULTS: The linear range of ginsenosides Rg1 and ginsenosides Re were 0.173～17.3 μg·mL-1 and 0.156～39.1 μg·mL-1 respectively. The precision and accuracy were in accordance with the requirements. CONCLUSION: The method is accurate, sensitive and specific for simultaneous determination of ginsenosides Rg1 and ginsenosides Re in total saponins of fibrous roots of P. notoginseng.