化学
喹喔啉
吲哚试验
色谱法
高效液相色谱法
固相萃取
三乙胺
喹啉
磷酸
萃取(化学)
乙腈
吡啶
溶剂
二氯甲烷
药物化学
有机化学
作者
Haitao Guo,Ping Han,Zhenyu Wang,Li Chen,Dequan Zhang
出处
期刊:Sepu
[China Science Publishing & Media Ltd.]
日期:2013-09-18
卷期号:30 (10): 1074-1080
被引量:4
标识
DOI:10.3724/sp.j.1123.2012.06023
摘要
A method was established for the simultaneous determination of nine heterocyclic aromatic amines (HAAs) in mutton products by solid phase extraction-high performance liquid chromatography (SPE-HPLC). As a result, the sample was prepared by ultrasound in 2 mol/L NaOH, and dichloromethane was selected as the extraction solvent. The extract was purified and concentrated with an MCX SPE column. The chromatographic separation was achieved on a reverse-phase C18 column by gradient elution using 0.01 mol/L phosphoric acid (adjusted to pH 3.6 by triethylamine) and acetonitrile, detected with a diode array detector (DAD) at 228 nm for 2-amino-9H-pyrido [ 2,3-b] indole (AaC) and 2-amino-3-methyl-9H-pyrido [ 2,3-b] indole (MeAaC); 253 nm for 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 1-methyl-9H-pyrido[3, 4-b] indole (Harman), 9H-pyrido[3,4-b] indole (Norharman); 263 nm for 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo [4,5-f] quinoxaline (4, 8-DiMeIQx), 3-amino-1-methyl-5H-pyrido [4,3-b] indole (Trp-p-2); 321 nm for 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP). The results showed that the nine HAAs can be separated efficiently. The recoveries (n = 6) of the nine HAAs spiked in meat were between 50.27% and 94.77% with the relative standard deviations of 0.08%-4.42%. The limits of detection of this method were 1.6 -41.0 microg/L for the nine HAAs. The method is simple, accurate, rapid and repeatable. It can be used for the simultaneous determination of the nine HAAs in mutton products.
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