Separation and Identification of Chelerythrine and Sanguinarine Monomer

Objective: To isolate and identify chelerythrine and sanguinarine monomer.Method: Chelerythrine and sanguinarine were isolated and purified by column chromatography technology,and identified by HPLC.Result: Single component could be obtained by column chromatography method,chelerythrine and the sanguinarine monomer were identified by HPLC with purity of 98.6%,95.7%,respectively.Conclusion: Column chromatography technology could obtain high purity chelerythrine and sanguinarine monomer from Macleaya cordata.