Study on the GDFN gene to transfect the OECs came from the surrounding tissue

OBJECTIVE To study GDFN gene to transfect the OECs came from the surrounding tissue,analyze the expression level of transfected cell,and to provide the basis for the gene therapy for SCI.MOTHODS We extracted the OECs came from the surrounding tissue,used recombination adenovirus as the vector,transfected the GDFN to the OECs,and analyzed the expression level of the transfected cell by using bio-medical method.RESULTS GDFN was transfected into OECs,the transfection efficiency was 77%,the expression level of transfected cell was 30.3 ng/106/24h,and the expression level was steady.CONCLUSION Gene therapy for SCI by using GDFN as target gene to transfect OECs was feasible.