Abnormal State of Coagulation in Experimental Colitis of Rats

Objective:To study the relationship between inflammatory activities and coagulation abnormal in the TNBS induced rat's colitis model. Method 30 SD-rats separated into 3 groups randomly,Include 1 normal control group: In this group every rats were given an enema with normal saline, and were killed 14 day after enema. 2 1week colitis group, in this group every rat was given an enema with TNBS, and was killed 7 day after enema. Colitis 2 weeks group: In this group every rats were given an enema with TNBS, and were killed 14 day after enema. We choose PT、APTT and the activity of AT as the index of coagulation. And we assess the level of damage and inflammatory state of the colitis rats by macroscopically score, histological score and the level of TNF-α in each group. Result Compared with normal control group, TNBS induced 1 week colitis group, and 2weeks colitis group has a shorter PT and APTT and a lower activity level of AT. The number of plate per litre is higher than the normal control group. The difference has statistical significance. And we find that index of tissue damage and inflammatory has negative correlation with PT 、APTT and AT. Conclusion There is abnormality of coagulation in the TNBS induced rat colitis,which is correspondence with the state of the illness closely. The test's result implied that abnormal state of coagulation in IBD patients have close correlation with the activity of inflammatory. And TNBS induced rat colitis model can act as a model of inflammation activate coagulation for further study in this field.