Butanol production from xylose fermentation in cellulose hydrolysate

A strain was obtained by screening with which butanol can be fermented by using cellulose hydrolysate. The microorganism not only could use glucose of cellulose hydrolysate,but also could use xylose of cellulose hydrolysate. The growth characteristics,carbon source,nitrogen source and CaCO3 addition in batch fermentation were studied. High concentration glucose and xylose could not be consumed. The optimal concentration of xylose was 20 g/L in batch fermentation. So the initial concentration of xylose in cellulose hydrolysate was 20 g/L with 15 L fermentation reactor,and then cultured for 84 hours at 37 ℃. The yield of butanol was 10.95 g/L,yield of total solvent 16.78 g/L(acetone,ethanol,and butanol),utilization rate of xylose was 70% and total solvent conversion was39.4%. The economic loss by failure of utilization of xylose in cellulose hydrolysate fermentation was resolved.