Preparation,Characterization and application of monoclonal antibodies to recombinant human interferon—α2a

In this report,five mouse hybridoma cell lines secreting monoclonal antibodies to recombi-nant human interferon—α2a(rHu IFN—α2a)were raised.All the monoclonal antibodies with IgGl subclass react with rHu IFN—α2a detected by indirect ELISA with the titers ranging from 1:320,000 to 1:10,240,000 but have no cross reactivity with rHu IFNαl,γ and proteins from recombinant E.coli.Two of monoclonal antibodies,designated 3F1 and 5G10,have the ability to neutralize antiviral activity of rHu IFN—α2a.By a competitive ELISA the five monoclonal anti-bodies can be divided into three categories based on their epitope specificities.A sandwich ELISA established by employing 3F1 and 5G10 monoclonal antibodies could detect rHuIFN-α2a or rHuIFN-α2b as few as 60pg/ml·Moreover,3F1 monoclonal antibody displayed a satisfactory property in affinity chromatography for rHuIFNα2a,which is able to match a monoclonal anti- body NK2 from CellTech Corporation.