Relationship between CCK-AR mRNA of gallbladder wall and plasma CCK-8 level and gallbladder emptying function in gallstone patients

[Objective] To investigate the relationship between the expression of cholecystokinin(CCK)-A receptor(AR)mRNA of gallbladder wall and the plasma CCK-8 level and emptying function of the gallbladder in gallstone patients. [Methods] The gallbladder emptying function was determined preoperatively with B ultrasonograph ,CCK-AR mRNA of gallbladder was studied by RT-PCR,plasma CCK-8 were detected by radioimmunoassay before the cholecystectomy and after the operation in 60 patients of gallstones group and 30 patients of control group. [Results] The CCK-AR mRNA of gallstones group was lower than that of the control group(P 0.01),and so did patients with impaired gallbladder motility than those with normal motility(P 0.01). Plasma CCK-8 level in gallstone group was significantly higher than that in control group (P 0.05). Plasma CCK-8 level before the cholecystectomy were significantly higher than that after the operation in the gallstone group (P 0.05). [Conclusion] CCK-AR and CCK-8 play an important role in the regulation of gallbladder motility.