Study on Extracting Process of Chlorogenic Acid from Flos Lonicerae

The extracting process of Chlorogenic acid from Flos Lonicerae was studied with different concentrations of ethanol. The processes were then optimized by orthogonal test, and Chlorogenic acid was determined by UV. The effects of concentration of ethanol, folds of ethanol, refluxing time, pH and extracting temperature on Chlorogenic acid content were studied. The result showed that the optimum extracting technology of Chlorogenic acid from Flos Lonicerae includes the extraction conditions of 10 folds of 50% ethanol , dip in 70℃ water , pH=7 , refluxing for 2 times and 1 h each time.