Research of Enzyme Hydrolysis of Collagen in Pig Skin to Prepare Antioxidative Peptides

The hydrolysis of pig skin collagen is carried out with pepsin digestion after denaturalization under high temperature. The antioxidative activity of the hydrolysate is determined with thiobarbituric acid reactive substances assay(TBA). The molecular weight of hydrolysate is detected with SDS-PAGE. The suitable hydrolysis condition is given as denaturalization time 5 min, ratio of pepsin 1.5%, total hydrolysis time is 0.5 h.