Optimization of SSR-PCR Non-denatured Polyacrylamide Gel Electrophoresis Conditions in Kernelled Apricot

[Objective] The aim was to optimize the SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot. [Method] 25 copies of kernelled apricot and three copies of edible apricot were selected as experimental materials to screen the repeatable SSR loci with high polymorphism by the use of SSR markers combined with non-denatured polyacrylamide gel electrophoresis. And the effect of different factors on electrophoresis conditions was compared to explore the optimization of SSR-PCR non-denatured polyacrylamide gel electrophoresis conditions in kernelled apricot. [Result] The optimization of non-denatured polyacrylamide gel electrophoresis conditions for SSR-PCR were established as follows: polyacrylamide gel concentration of acrylamide 6%,the ratio of acrylamide to bisacrylamide 29∶1,electrophoresis at 1 000 V for 2-3 h,and staining 15 min in 0.1% AgNO3. [Conclusion] The optimum electrophoresis system had provided some technical foundations to study the phylogenetic relationship of kernelled apricots by SSR marker.