Hairy Root Induced by Agrobacterium rhizogenes and the Application in Plant Science

Agrobacterium rhizogenes contains Ri plasmid,the rolBgenes is on the T-DNA of Ri plasmid,rolB genes can form a lot of hairy root through the wound infection of Agrobacterium rhizogenes.By the transformation of hairy root can get regenerate plants which show many stabilizes in the phenotypic variation of the genetic,so it has a broad application prospect in plant genetic improvement.The biological characteristics of Agrobacterium rhizogenes and the structure and function of Ri plasmid were summarized,as well as rolBgene loci and characteristics,Agrobacterium rhizogenes transformation mechanism and the methods of the hairy root induction,the influence factors of inducing hairy root,identifying and hairy plantlet regeneration after the eradication of bacteria and so on.In addition,the application in plant sciences was reviewed.