Determination of Drug-loading Rate and Encapsulation Efficiency of Quercetin Solid Lipid Nanoparticles

OBJECTIVE:To establish the determination method of the drug-loading rate and encapsulation efficiency of Quercetin solid lipid nanoparticles(QUE-SLNs).METHODS:A high-velocity centrifuge-HPLC was adopted.The determination was performed on DiamonsiL-C18(250 mm×4.6 mm,5 μm)column with mobile phase consisted of methanol-4.3% acetic acid solution(55 :45) at flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm and column temperature was 30 ℃.RESULTS:The linear range of quercetin was 2.0～200.0 μg·mL-1(r=0.999 6) with an average recovery of 97.83%(RSD=1.03%,n=6).The encapsulation efficiency of QUE-SLNs was 80.2% and the drug-loading rate was 1.7%.CONCLUSION:The method is simple,convenient and reliable.It can be used for the determination of drug-loading rate and encapsulation efficiency of SLN.