Objective:To study the effect of bifidobacterium on irritable bowel syndrome(IBS).Methods:Eight-four patients with IBS were administered 3-4 pieces of bifidobacterium each time for three times per day and the symptoms were assessed every week before and after bifidobacterium therapy.Results:The total response rates of two and four weeks after the therapy 63.38% and 83.40%,respectively.The effect was more obvious on mitigating of abdominal pain,abdominal distension,abdominal tone and diarrhea.Conclusions:Bifidobacterium is effective for treatment of IBS syndrome with little side effect.