Nineteen differential lines possessing known barley powdery mildew(Blumeria graminis f.sp.hordei)resistant genes were used in virulence analysis for the population consisted of 213 isolates collected from four areas along the southeast coastal region of China.Most of virulent genes investigated in this study showed very similar virulence frequencies in the four different areas.Among these known resistant genes,Mla11,Mlmw,Mlkb,Mlmlw,Mlk2,(Mla10 MlDu2)and(MlK1 Mla8)were found as ones effective for powdery mildew resistance breeding in all the four areas,while Mla8,Mlnn,Mlh,(Mlra Mla8),(MlRu2 Mla8),(MlBw Mla8),(Mlra MlDr2)and(MlLa Mla8)as ineffective ones.