HPLC determination of salidroside in 6 rhodiola species produced in qinghai

OBJECTIVE To study the extraction method and the HPLC determination conditions and to determine salidroside in Rhodiola kirilowii,R.algida,R.coccinea,R.quadrifida,R.crenulata and R.juparesis.METHODS With a Spherisorb C 18 (5 μm) column (4.6 mm×250 mm),MeOHH 2O(20∶80) as mobile phase,detected at 223 nm.RESULT Linear range of salidroside was 0.097～0.87 μg.MeOH extraction time was 30 min.The recovery rate was 99.6%(RSD=1.62%).CONCLUSION This method provides a simple and feasible way to determine salidroside and the salidroside content was different in different rhodiola species.