Optimization of Fermentation Conditions for 2,3-Butanediol from Xylose

The production of 2,3-butanediol was carried out from fermentation of xylose by Klebsiella pneumoniae.The effects of pH,temperature,amount of medium and rotation on 2,3-butanediol were studied by the single-factor tests and L9(34)orthogonal design experiment.The parameters of optimum fermentation were suggested as follows: xylose mass concentration 80 g/L,inoculum 6 %(volume fraction),initial pH value 6.0,rotation120 r/min,amount of medium 80 mL/250 mL and 35 ℃,concentration of 2,3-butanediol reached 33.0 g/L and yield of 2,3-butanediol from xylose was 0.46 g/g.