生化工程
农药残留
适体
生物技术
杀虫剂
生物传感器
食品安全
计算机科学
化学
纳米技术
生物
工程类
食品科学
材料科学
农学
遗传学
作者
Mingqi Zhao,Miao Wang,Xiaoguang Zhang,Yongan Zhu,Jing Cao,Yongxin She,Zhen Cao,Guangyue Li,Jing Wang,A. M. Abd El‐Aty
标识
DOI:10.1080/10408398.2021.2009762
摘要
Excessive use of pesticides can cause contamination of the environment and agricultural products that are directly threatening human life and health. Therefore, in the process of food safety supervision, it is crucial to conduct sensitive and rapid detection of pesticide residues. The recognition element is the vital component of sensors and methods for fast testing pesticide residues in food. Improper recognition elements may lead to defects of testing methods, such as poor stability, low sensitivity, high economic costs, and waste of time. We can use the molecular biological technique to address these challenges as a good strategy for recognition element production and modification. Herein, we review the molecular biological methods of five specific recognition elements, including aptamers, genetic engineering antibodies, DNAzymes, genetically engineered enzymes, and whole-cell-based biosensors. In addition, the application of these identification elements combined with biosensor and immunoassay methods in actual detection was also discussed. The purpose of this review was to provide a valuable reference for further development of rapid detection methods for pesticide residues.
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