核糖核酸
质量细胞仪
单细胞分析
转录组
免疫系统
单细胞测序
计算机科学
作者
Li Song,David M. Cohen,Zhangyi Ouyang,Yang Cao,Xihao Hu,X. Shirley Liu
出处
期刊:Nature Methods
[Springer Nature]
日期:2021-05-13
卷期号:18 (6): 627-630
被引量:6
标识
DOI:10.1038/s41592-021-01142-2
摘要
We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in αβ/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer—even full-length—receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5′ 10x Genomics platforms. TRUST4 is a computational tool for reconstructing T-cell and B-cell receptor repertoires using bulk and single-cell RNA-seq data.
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