1H NMR-based metabolomics and lipidomics of microalgae

1H NMR-based metabolomics and lipidomics is a robust, nondestructive, reproducible, and high-throughput technique that allows rapid identification and quantification of large numbers of metabolites without requiring any prior information about the source organism (i.e., microalgae). It is an effective tool to identify and decipher complex alterations in the microalgae in response to different environmental stressors. 1H NMR data can be processed using spectral databases and statistical software for identification and quantification of differentially expressed metabolites and lipids. This knowledge can then be leveraged to enhance the desired metabolite and/or lipid in the target microalgal strain(s).