乳腺癌
小RNA
生物标志物
液体活检
细胞外小泡
阶段(地层学)
三阴性乳腺癌
癌症
癌症生物标志物
胞外囊泡
癌症研究
医学
肿瘤科
接收机工作特性
微泡
内科学
生物
基因
遗传学
细胞生物学
古生物学
作者
Min Woo Kim,Sunyoung Park,Hyojung Lee,Hogyeong Gwak,Kyung-A Hyun,Jee Ye Kim,Hyo-Il Jung,Kyung Sik Kim
出处
期刊:Cancer Science
[Wiley]
日期:2021-10-27
卷期号:112 (12): 5078-5087
被引量:29
摘要
Extracellular vesicles (EV) have been emerging as potential biomarkers for disease monitoring. In particular, tumor-derived EV (TDE) are known to carry oncogenic miRNA, so they can be used for diagnosis of early cancer by analyzing the expression levels of EV-miRNA circulating in the blood. Here, using our novel microfluidic device, we rapidly and selectively isolate cancerous EV expressing breast cancer-derived surface markers CD49f and EpCAM within 2 minutes. Based on seven candidates of miRNA nominated from The Cancer Genome Atlas (TCGA) database, the expression levels of miRNA in TDE were validated in a total of 82 individuals, including 62 breast cancer patients and 20 healthy controls. Among seven candidates, four miRNAs (miR-9, miR-16, miR-21, and miR-429) from the EV were highly elevated in early-stage breast cancer patients compared with healthy donors. The combination of significant miRNAs from specific EV has high sensitivities of 0.90, 0.86, 0.88, and 0.84 of the area under the receiver operating characteristic curve (AUC) in each subtype (luminal A, luminal B, HER-2, and triple-negative) of early-stage breast cancer. Our results suggest that the combination of four miRNA signatures of specific EV could serve as a sensitive and specific biomarker and enable early diagnosis of breast cancer using liquid biopsy.
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