Sequencing and bioinformatic analysis of mRNA from Echinococcus granulosus protoscolex

Objective To reveal the transcriptomic information and biological characteristics of Echinococcus granulosus protoscolex by using RNA-Seq technique and through bioinformatic analysis of sequencing data.Methods Total RNA was isolated from Echinococcus granulosus protoscolex using TRIzol.mRNA kit with Oligo(dT) magnetic beads was used to isolate poly(A) mRNA.Illumina HiSeq 2000 was applied for sequencing.Data from sequeced were assembled into unigene by using mapping-first approaches based on the genome which uploaded in the Wellcome Trust Sanger Institute.Alignment between unigenes and protein databases,such as NCBI non-redundant protein (Nr) database,UniProt database,the gene ontology (GO) database,the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database,was performed.Results A total of 132 007 609 clean reads and 91 342 unigenes were generated.The average length of unigene was 419 bp.Through analysed with GO,26 552 unigenes were mapped.Further biological process categories of GO prominently represented in biological process,molecular functions and cellular components including 48 categories.Six thousand six hundred and sixty-four unigenes were mapped to 227 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways,including 6 first level categories and 34 second level categories.Conclusion Extensive transcriptome data from sequencing of Echinococcus granulosus protoscolex were obtained.The information has provided a database for further study on the hydatid disease. Key words: Echinococcus granulosus protoscolex;  Transcriptome;  RNA-Seq;  Bioinformatics analysis