纳米探针
化学
胶体金
肽
纳米技术
组合化学
纳米颗粒
细胞内
荧光
色谱法
生物化学
材料科学
物理
量子力学
作者
Wei Pan,Xiaohan Liu,Xiuyan Wan,Jia Li,Yanhua Li,Fei Lü,Na Li,Bo Tang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2019-11-18
卷期号:91 (24): 15982-15987
被引量:15
标识
DOI:10.1021/acs.analchem.9b04616
摘要
Fluorescent nanoprobes based on peptide-functionalized gold nanoparticles (AuNPs) have been widely used in bioassays. The Au–Se bond is considered as a better candidate than the Au–S bond to link the peptides and AuNPs due to the stronger ability against interference of intracellular thiol. However, the current synthetic methods for preparing peptide/AuNPs nanoprobes are always complex and time-consuming. Developing a convenient and rapid method to synthesize the Au–Se bond based nanoprobes is expected to further facilitate their application in fundamental research. Herein, we present a facile and rapid approach to prepare the Au–Se-peptide nanoprobes through a direct freezing process, which is easy-to-operate, time-saving, and surfactant-free. Compared with the traditional method, the amount of peptide loaded on AuNPs by freezing method is also promoted with 20–30%. Furthermore, the obtained nanoprobe was successfully applied to identify autophagy and apoptosis in chemotherapeutic drug treated cancer cells.
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