体内
体外
肿瘤坏死因子α
一氧化氮
凝胶渗透色谱法
化学
药理学
生物活性
生物
免疫学
生物化学
生物技术
内分泌学
有机化学
聚合物
作者
Huimei Wang,Xuelian Wang,Ying Liu,Shaojie Zhang,Zhengguo Li,Yuhao Li,Jianlin Cui,Xiaozhong Lan,Erhao Zhang,Lei Yuan,Da-Qing Jin,Muhetaer Tuerhong,Munira Abudukeremu,Jing Xu,Yuanqiang Guo
标识
DOI:10.1016/j.carbpol.2020.117521
摘要
In our continuous searching for natural active polysaccharides with immunomodulatory activity, an arabinofuranan (AQP70-3) was isolated and purified from the fruits of Akebia quinata (Houtt.) Decne. by using ion-exchange chromatography and gel permeation chromatography for the first time. AQP70-3 contained both α-l-Araf and β-l-Araf, and the absolute molecular weight was 1.06 × 104 g/mol. The backbone of AQP70-3 comprised →5)-α-l-Araf-(1→, →3,5)-α-l-Araf-(1→, and →2,5)-α-l-Araf-(1→, with branches of →1)-β-l-Arafand →3)-α-l-Araf-(1→ residues. Biological assay suggested that AQP70-3 can stimulate phagocytic activity and promote the levels of nitric oxide (NO), interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) of RAW264.7 cells. Furthermore, AQP70-3 was found to increase the production of reactive oxygen species (ROS) and NO in zebrafish embryo model.
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