毛皮
化学
共域化
高尔基体
费斯特共振能量转移
荧光
生物物理学
细胞生物学
生物化学
光学
酶
细胞
生物
物理
作者
Xiao Hu,Zijuan Hai,Chengfan Wu,Wenjun Zhan,Gaolin Liang
标识
DOI:10.1021/acs.analchem.0c04186
摘要
Development of fluorescence probes for highly accurate detection of cancer-related enzyme activity is important in early cancer diagnosis. Herein, we report a Golgi-targeting and dual-color “Turn-On” probe Q-RVRR-DCM for imaging furin with high spatial precision. By integrating the principles of Förster resonance energy transfer and intramolecular charge transfer, the probe was designed to be non-fluorescent. Upon furin cleavage, Q-RVRR-DCM was converted into Q-RVRR and DCM-NH2, turning the dual fluorescence color “On” at 420 and 640 nm without spectral cross-talk. In furin-overexpressing HCT116 cells, Q-RVRR-DCM showed not only furin-specific, dual-color “Turn-On” fluorescence but also superior colocalization with a Golgi tracker than the single-color “Turn-On” probe RVRR-DCM. We envision that, with the excellent properties of Golgi-targeting and dual fluorescence color “Turn-On”, our furin probe Q-RVRR-DCM could be applied for accurate early diagnosis of cancer in the near future.
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