High Performance Liquid Chromatographic Preparation of Alternariol, Alternariol Methyl Ether, and Altenuene

Abstract A solvent system was developed for the isolation of 3 major Alternaria mycotoxins by high performance liquid chromatography (HPLC). Alternariol (AOH), alternariol methyl ether (AME), and altenuene (ALT) were purified using either a semipreparative or a preparative HPLC column. Gram quantities of pure mycotoxins were obtained in a single preparative HPLC step. Distribution of other individual mycotoxins and metabolites in various fractions obtained from either chromatographic procedure is discussed.