癌基因
强直性脊柱炎
癌症研究
Wnt信号通路
连环素
分子医学
骨化
间质细胞
细胞周期
细胞周期蛋白D1
CXCR4型
细胞生长
生物
病理
医学
化学
内科学
炎症
免疫学
趋化因子
细胞生物学
信号转导
解剖
癌症
生物化学
作者
C. He,LI Da-he,Jian‐Zhong Gao,Jia Li,Zhongtang Liu,Weidong Xu
出处
期刊:Molecular Medicine Reports
[Spandidos Publications]
日期:2019-02-22
被引量:9
标识
DOI:10.3892/mmr.2019.9980
摘要
Ankylosing spondylitis (AS) is an autoimmune condition characterized by chronic inflammation and abnormal ossification as the primary features of the disease. The aim of the present study was to investigate the role of C‑X‑C chemokine receptor type 4 (CXCR4) in ossification from patients with AS. CXCR4 expression was assessed by western blot analysis and immunohistochemistry analysis of tissues obtained from patients with AS and controls. Fibroblasts were isolated, cultured and incubated with AMD 3100 and stromal cell‑derived factor‑1 to inhibit and promote CXCR4 levels, respectively. CXCR4 was upregulated in hip synovial tissues from patients with AS compared with that observed in controls. AS fibroblasts exhibited increased proliferation and growth rates. Inhibition of CXCR4 increased the phosphorylation of β‑catenin and downregulated the expression of β‑catenin, v‑myc avian myelocytomatosis viral oncogene homolog, cyclin D1 and osteocalcin. Alizarin red staining demonstrated a decrease in biomineralization activity following the inhibition of CXCR4. These data support the hypothesis that inhibiting CXCR4 in patients with AS may suppress the ossification of fibroblasts.
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