坦克结合激酶1
先天免疫系统
生物
基因敲除
细胞生物学
干扰素
仙台病毒
小干扰RNA
蛋白激酶R
病毒学
病毒
分子生物学
激酶
核糖核酸
蛋白激酶A
遗传学
基因
受体
MAP激酶激酶激酶
丝裂原活化蛋白激酶激酶
作者
Kexin Hua,Yaqian Li,Hongjian Chen,Jiamin Ni,Dingren Bi,Rui Luo,Hui Jin
出处
期刊:Cytokine
[Elsevier]
日期:2018-11-01
卷期号:111: 325-333
被引量:10
标识
DOI:10.1016/j.cyto.2018.09.007
摘要
TRAF family member-associated NF-κB activator (TANK)-binding kinase 1 (TBK1) serves as hub molecule at the crossroad of multiple signaling pathways of type I interferon (IFN) induction. The importance of TBK1 in innate immunity has been demonstrated in mammalian, however the characterization and function of TBK1 in avian remains largely unknown. In this study, we cloned duck TBK1 (duTBK1) from duck embryo fibroblasts (DEFs) for the first time, which encoded 729 amino acids and had a high amino acid identity with goose and cormorant TBK1s. The duTBK1 showed a diffuse cytoplasmic localization in DEFs and was extensively expressed in all tested tissues. Overexpression of duTBK1 induced IFN-β production through the activation of IRF1 and NF-κB in DEFs. The N-terminal kinase domain and the ubiquitin-like domain in middle of duTBK1 played pivotal roles in IFN-β induction as well as in IRF1 and NF-κB activation. Furthermore, knockdown of duTBK1 by small interfering RNA significantly decreased poly(I:C)- or Sendai virus (SeV)-induced IFN-β expression. In addition, duTBK1 expression dramatically reduced the replication of both duck reovirus (DRV) and duck Tembusu virus (DTMUV) in DEFs. These results suggested that the duTBK1 played a pivotal role in mediating duck antiviral innate immunity.
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