Evaluation of spheroid form properties by the visualization of the image levels distribution

This research group has established a technique to make a three-dimensional cell construct using only cells. The cell construct made uses cultured spheroids and the quality of the spheroids is important, it is necessary to provide reproducible spheroids. Therefore, it is necessary to morphology-evaluation spheroids. The quality of spheroids was evaluated by area, diameter, and circularity. However, the concentration distribution of the spheroids had not ever been visualized. This study aimed to grasp the concentration distribution visually using pictures of the spheroids and observed serial changes of spheroid's concentration distribution. It could grasp visually the spheroid's concentration distribution. We thought the spheroid serially condense cells and density was high at the same time. However it could confirm a part of low density. It is considered that the change which the cells move in the spheroid occur.