A rational design of ratiometric fluorescent probes based on new ICT/FRET platform and imaging of endogenous sulfite in living cells

A new ICT/FRET platform composed of coumarin-hemicyanine dyad was rationally designed for ratiometric fluorescent probes. The platform could concert the spectral overlap and the emission shifts by changing ICT donor. The mitochondria-targeting probe was developed to sense endogenous sulfite in living cells with high selectivity, sensitivity and fast response.