Chitosan/glycyrrhizic acid hydrogel: Preparation, characterization, and its potential for controlled release of gallic acid

In the present work, chitosan (CHT) as a biodegradable polymer was crosslinked using various amounts of glycyrrhizic acid (GLA) as a novel crosslinking agent to prepare biocompatible hydrogels. The prepared hydrogels were used for the controlled release of gallic acid (GA) in transdermal therapy application. FTIR, XRD, and SEM were used to characterize the prepared gels. The results indicated that the carboxylic acid groups of GLA react with the amine groups of the CHT in the presence of activating coupling reagents to form covalent amide linkage between the polymer chains of CHT and construct CHT cross-linked hydrogel (CCH) network structure. The prepared CCH samples were characterized and used for the controlled release of a drug, i.e. (GA). For this purpose, the swelling kinetic, loading and encapsulation efficiency, in vitro drug release, drug release kinetics, cell viability assay, and anti-bacterial activity of the samples were evaluated. The swelling ratio of CCH samples were in the range of 455-37 % depending on the pH of environment. Swelling kinetic results showed an aggregate to the non-linear second-order kinetic model. Drug release results were fitted by kinetic models while the Korsmeyer-Peppas model was fitted better. The CCH samples exhibited high biocompatibility for 5 mg/ml hydrogel concentration. In addition, the CHT and CCH sample without the GA did not show anti-bacterial properties for 1200 and 150 μg/ml concentrations, respectively. The CCH sample containing the GA exhibited enough anti-bacterial activity on the S. aureus bacteria strain at 150 μg/ml concentration. In contrast, the CCH sample containing the GA has a light anti-bacterial effect on the E. coli bacteria strain. The calculated mesh size of hydrogel networks, drug size, and kinetics models revealed that the CCH samples could release GA based on a diffusion mechanism. In conclusion, the designed CCH samples have enough ability for controlled drug release in transdermal applications.