Investigating the neurotoxicity of environmental pollutants using zebrafish as a model organism: A review and recommendations for future work

With the continuous development of precise detection technology, more and more pollutants have been detected in the environment. Among them, neurotoxic pollutants have attracted extensive attention due to their serious threat to vertebrates, invertebrates, and the whole ecosystem. Compared with other model organisms, zebrafish (Danio rerio) have become an important aquatic model to study the neurotoxicity of environmental pollutants because of their excellent molecular/physiological characteristics. At present, the research on the toxicity of environmental pollutants to the zebrafish nervous system focuses on morphology and behavior regulation, oxidative stress, gene expression, synthesis and release of neurotransmitters, and neuron development. However, studies on epigenetic toxicity, blood-brain barrier damage, and regulation of the brain-gut-microbiota axis still require further research at the molecular and signaling levels to clarify the toxic mechanisms of pollutants. This paper reviews the research on the toxic effects of pollutants in the environment (heavy metals and organic compounds) on the nervous system of zebrafish, summarizes and comments on the main research findings. The discussion of the problems, hot spots in the current research, and the prospects of the contents to be further studied are also included in this paper.