小干扰RNA
透明质酸
内吞作用
体内
CD44细胞
体外
基因沉默
PLK1
壳聚糖
癌细胞
癌症研究
化学
细胞生物学
细胞
细胞周期
癌症
生物
转染
生物化学
遗传学
生物技术
基因
作者
Maryamsadat Shahidi,Omid Abazari,Parisa Dayati,Javad Zavar Reza,Mohammad Hossein Modarressi,Davood Tofighi,Bibi Fatemeh Haghiralsadat,Fatemeh Oroojalian
出处
期刊:Nanomedicine
日期:2023-02-01
卷期号:18 (3): 259-277
被引量:1
标识
DOI:10.2217/nnm-2022-0198
摘要
Aims: Achieving an effective biocompatible system for siRNAs delivery to the tumor site remains a significant challenge. Materials & methods: Selenium nanoparticles (SeNPs) modified by chitosan (CS) and hyaluronic acid (HA) were fabricated for PLK1 siRNAs ( siPLK1) delivery to the bladder cancer cells. The HA-CS-SeNP@siPLK1 efficacy was evaluated using in vitro and in vivo models. Results: HA-CS-SeNP@siPLK1 was selectively internalized into T24 cells through clathrin-mediated endocytosis. Treatment with HA-CS-SeNP@siPLK1 successfully silenced the PLK1 gene, inhibited cell proliferation and induced cell cycle arrest in vitro. HA-CS-SeNP@siPLK1 could also inhibit tumor growth in vivo without causing systemic toxicity. Conclusion: Our results suggest that HA-CS-SeNPs may provide a good vehicle for delivering siPLK1 to the bladder tumor site.
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