Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production

Abstract Osmotic stress, caused by the lack of water or by high salinity, is a common problem in plant roots. Osmotic stress can be reproducibly simulated with the application of solutions of the high-molecular-weight and impermeable polyethylene glycol. The accumulation of different reactive oxygen species, such as singlet oxygen, superoxide, and hydrogen peroxide, accompany this stress. Among them, singlet oxygen, produced as a byproduct of lipoxygenase activity, has been associated with limiting root growth. To better understand the source and effect of singlet oxygen, we followed its production at the cellular level in Arabidopsis (Arabidopsis thaliana). Osmotic stress initiated profound changes in plastid and vacuole structure. Confocal and electron microscopy showed that the plastids were a source of singlet oxygen accompanied by the appearance of multiple, small extraplastidic bodies that were also an intense source of singlet oxygen. A marker protein, CRUMPLED LEAF, indicated that these small bodies originated from the plastid outer membrane. Remarkably, LINOLEATE 9S-LIPOXYGENASE 5 (LOX5) was shown to change its distribution from uniformly cytoplasmic to a more clumped distribution together with plastids and the small bodies. In addition, oxylipin products of Type 9 lipoxygenase increased, while products of Type 13 lipoxygenases decreased. Inhibition of lipoxygenase by the salicylhydroxamic acid inhibitor or in downregulated lipoxygenase lines prevented cells from initiating the cellular responses, leading to cell death. In contrast, singlet oxygen scavenging halted terminal cell death. These findings underscore the reversible nature of osmotic stress-induced changes, emphasizing the pivotal roles of lipoxygenases and singlet oxygen in root stress physiology.