生物
遗传学
基因
系统发育树
非生物胁迫
转录因子
藜藜
同源(生物学)
基因家族
系统发育学
保守序列
计算生物学
基因组
植物
肽序列
作者
Guoxing Xue,Yue Fan,Changjie Zheng,Hao Yang,Liang Feng,Xingyu Chen,Yanjie Yang,Xin Yao,Wenfeng Weng,Lingyan Kong,Chuang Liu,Jianping Cheng,Jingjun Ruan
标识
DOI:10.3389/fpls.2023.1171518
摘要
The second-largest transcription factor superfamily in plants is that of the basic helix-loop-helix (bHLH) family, which plays an important complex physiological role in plant growth, tissue development, and environmental adaptation. Systematic research on the Chenopodium quinoa bHLH family will enable a better understanding of this species. Herein, authors used a variety of bioinformatics methods and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to explore the evolution and function of the 218 CqbHLH genes identified. A total of 218 CqbHLH transcription factor genes were identified in the whole genome, located on 18 chromosomes. A phylogenetic tree was constructed using the CqbHLH and AtbHLH proteins to determine their homology, and the members were divided into 20 subgroups and one unclustered gene. Authors also analyzed 218 CqbHLH genes, conservative motifs, chromosome diffusion, and gene replication. The author constructed one Neighbor-Joining (NJ) tree and a collinearity analysis map of the bHLH family in C. quinoa and six other plant species to study the evolutionary relationship and homology among multiple species. In addition, the expression levels of 20 CqbHLH members from different subgroups in various tissues, different fruit developmental stages, and six abiotic stresses were analyzed. Authors identified 218 CqbHLH genes and studied their biological functions, providing a basis for better understanding and further studying the bHLH family in quinoa.
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