软骨发生
自愈水凝胶
间充质干细胞
细胞生物学
化学
糖胺聚糖
软骨细胞
细胞外基质
细胞分化
干细胞
生物物理学
体外
生物
生物化学
高分子化学
基因
作者
Hanwen Cao,Zhulian Li,Yafang Chen,Jiayi Zhu,Manyu Chen,Haoyuan Lei,Yun Xiao,Jie Liang,Tun Yuan,Yong Sun,Yujiang Fan,Xingdong Zhang
标识
DOI:10.1016/j.compositesb.2023.110871
摘要
Chondrogenic differentiation of stem cells can be regulated by mechanical properties of biomaterials. Here, hybrid hydrogels, composed of sulfhydrylated hyaluronan (HA-SH) with different molecular weight and collagen I (Col Ⅰ), were designed to investigate chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs). The enhanced molecular weight of HA-SH decreased the mechanical properties of hybrid hydrogels, but improved viscosity and degradation resistance, which could modulate cell spreading and morphological changes, thereby influencing chondrogenic differentiation. Furthermore, 1 MDa HA-SH combined with Col Ⅰ (1M-HAC) with adequate viscoelasticity was beneficial to the circularity and nuclear chromatin condensation of rBMSCs, which induced significant chondrocyte aggregation, increased glycosaminoglycans and collagen Ⅱ stainings, and reduced collagen Ⅹ staining. This was conducive to preserve a hyaline cartilage's phenotype and reducing inflammation and degeneration. Subcutaneous implantation studies revealed that 1M-HAC also facilitated specific matrix secretion, chondrogenic gene expression and potentially anti-inflammatory and anti-degenerative effects.
科研通智能强力驱动
Strongly Powered by AbleSci AI