精子
化学
输卵管
人类受精
细胞生物学
生物物理学
微流控
纳米技术
解剖
男科
生物
材料科学
内分泌学
医学
作者
Sai‐Xi Yu,Yi Wu,Guangyin Jing,Yanan Liu,Yu‐Chen Chen,Yajun Wang,Wei Liu,Jianan Tang,Huijuan Shi,Huafang Gao,Guangyin Jing,Yan‐Jun Liu
标识
DOI:10.1021/acs.analchem.2c04338
摘要
Before fertilization, sperms adhere to oviductal epithelium cells, and only a restrictive number of winner sperms can escape to reach the egg. To study the sperm escape behavior from the oviductal surface, we developed a microfluidic chip to fabricate an adhesive surface and to create a gradient of progesterone (P4) for mimicking the oviduct microenvironment in vivo. We identified three sperm motion patterns in such a microenvironment─anchored spin, run-and-spin, and escaped mode. By using kinetic analysis, we verified the hypothesis that the responsive rotation energy anchored with the adhered sperm head determines whether the sperm is trapped or detaching, which is defined as the hammer flying strategy of successful escape after accumulating energy in the process of rotating. Intriguingly, this hammer-throw escaping is able to be triggered by the P4 biochemical stimulation. Our results revealed the tangled process of sperm escape before fertilization in the ingenious microfluidic system.
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