Novel N-doped carbon dots derived from citric acid and urea: fluorescent sensing for determination of metronidazole and cytotoxicity studies

Blue emitting nitrogen-doped carbon dots were synthesized using citric acid and urea through the hydrothermal method, and the fluorescence quantum yield was 35.08%. We discovered that N-CDs featured excellent robust fluorescence stability and chemical resistance. For metronidazole detection, our N-CDs exhibited quick response time, high selectivity and sensitivity, and low cytotoxicity. Specifically, our N-CDs could detect metronidazole in the linear range of 0-179 μM, and the LOD was 0.25 μM. Furthermore, metronidazole efficaciously quenches the fluorescence of N-CDs, possibly owing to the inner filter effect. Lastly, we have employed our N-CDs to detect metronidazole in commercial metronidazole tablets with high accuracy. Overall, the newly prepared fluorescence sensor, N-CDs, demonstrated a huge potential to detect metronidazole in a simple, efficient, sensitive, and rapid manner.