Refining antibiotic cocktail regimens for pseudo-germ-free mice and their impact on gut microbiome and pancreatic tumor proteomics

Refined antibiotic regimens effectively generate PGF mice with minimized side effects, enabling reproducible studies of microbiota-host interactions. Microbiota depletion alters the tumor proteomic landscape and improves chemotherapy response in PDAC, highlighting the therapeutic potential of microbiome manipulation in cancer treatment.