Glycerol‐Assisted DNA Self‐Assembly at Low Temperatures

Abstract In preparing DNA nanostructures, thermal annealing is a common procedure to direct the proper DNA self‐assembly. However, the denaturing condition is not ideal in biomolecular applications in which physiological or lower temperature is preferred. In this study, glycerol is supplemented in the reaction system for DNA self‐assembly at arbitrary temperatures. The simple implementation leads to the successful generation of a variety of synthetic constructs. Notably, the glycerol‐based DNA nanostructure production sets the assembly temperature to an unprecedented freezing level. The co‐assembly test with guest protein from cell lysate shows a perfect compatibility of the method with the functioning of natural biomolecules and points to the emerging opportunities in molecular and cellular biology applications.